Fabrication of highly porous poly (ɛ-caprolactone) fibers for novel tissue scaffold via water-bath electrospinning

Highly porous fibers were prepared by water-bath electrospinning from pure poly(ɛ-caprolactone) (PCL), and its blends with methoxy poly(ethylene glycol) (MPEG). These fibers were further analyzed by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), X-ray diffraction (XRD), and gravimetric as well as contact angle measurement. SEM images showed that the fibers diameters as well as pores diameter on the fibers were affected by the weight ratio of MPEG/PCL. DSC and XRD not only revealed suppression of crystallinity of PCL but also indicated the presence of trace amount of MPEG in PCL water-bath collected fibers. The potential use of these hydrophilic porous electrospun fibrous mats as scaffolding materials was evaluated in vitro using mouse osteoblasts (MC3T3-E1) as reference cell lines. Cytotoxicity assessment of the fiber mats indicated that the porous electrospun mat containing trace amount of MPEG was nontoxic to the cell. Cell culture results showed that porous fibrous mats were good in promoting the cell attachment and proliferation. This novel electrospun matrix could be used as potential tissue scaffold material.

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► Highly porous PCL microfibers were obtained from water-bath electrospinning of blend solutions of PCL and MPEG.
► The pore size and depth on the fibers depended on the ratio of PCL/MPEG in the blends.
► Porous hybrid PCL fibrous mat showed lower crystallinity and higher hydrophilicity than that of conventional PCL fibers.
► Better cell attachment and proliferation on porous fibers than that of nonporous one.