کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6022177 | 1580661 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Blocked angiogenesis in Galectin-3 null mice does not alter cellular and behavioral recovery after middle cerebral artery occlusion stroke
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کلمات کلیدی
ECMβ-catphosphorylated histone H3PECAM-1SVZ5-bromo-2-deoxyuridineMCAONeuNDcxCBFRMSPCPGFAPMCAAngiogenesis - آنژیوژنزAcetylated tubulin - اتوپولین آستیلتیکmiddle cerebral artery occlusion - انسداد شریان (سرخرگ) مغزی میانیIschemia - ایسکمیHypoxia ischemia - ایسکمی هیپوکسیβ-catenin - بتا-کاتنینBrdU - بروموداکسی اوریدینanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceTerminal deoxynucleotidyl transferase dUTP nick end labeling - ترمینال deoxynucleotidyl transferase dUTP نام نهایی پایان نامهNeural repair - تعمیر عصبیTUNEL - تونلcerebral blood flow - جریان خون مغزیrostral migratory stream - جریان مورانو روسترالdoublecortin - دوچرخهH/I - سلامmiddle cerebral artery - شریان مغزی میانیVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)Planar Cell Polarity - قطبش پلانارExtracellular matrix - ماتریکس خارج سلولیCSF - مایع مغزی نخاعیCerebrospinal fluid - مایع مغزی نخاعیsubventricular zone - منطقه فرعیMigration - مهاجرتplatelet endothelial cell adhesion molecule-1 - مولکول چسبندگی سلول اندوتلیال پلاکتی-1Subependymal zone - ناحیه SubependymalNeurogenesis - نوروژنزwild type - نوع وحشیneuronal nuclei - هسته های نورونیGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالolfactory bulb - پیاز بویاییGal-3 - گال سومGalectin-3 - گالکتین-3gamma-tubulin - گاما توبولین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
عصب شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Angiogenesis is thought to decrease stroke size and improve behavioral outcomes and therefore several clinical trials are seeking to augment it. Galectin-3 (Gal-3) expression increases after middle cerebral artery occlusion (MCAO) and has been proposed to limit damage 3Â days after stroke. We carried out mild MCAO that damages the striatum but spares the cerebral cortex and SVZ. Gal-3 gene deletion prevented vascular endothelial growth factor (VEGF) upregulation after MCAO. This inhibited post-MCAO increases in endothelial proliferation and angiogenesis in the striatum allowing us to uniquely address the function of angiogenesis in this model of stroke. Apoptosis and infarct size were unchanged in Gal-3â/â mice 7 and 14Â days after MCAO, suggesting that angiogenesis does not affect lesion size. Microglial and astrocyte activation/proliferation after MCAO was similar in wild type and Gal-3â/â mice. In addition, openfield activity, motor hemiparesis, proprioception, reflex, tremors and grooming behaviors were essentially identical between WT and Gal-3â/â mice at 1, 3, 7, 10 and 14Â days after MCAO, suggesting that penumbral angiogenesis has limited impact on behavioral recovery. In addition to angiogenesis, increased adult subventricular zone (SVZ) neurogenesis is thought to provide neuroprotection after stroke in animal models. SVZ neurogenesis and migration to lesion were overall unaffected by the loss of Gal-3, suggesting no compensation for the lack of angiogenesis in Gal-3â/â mice. Because angiogenesis and neurogenesis are usually coordinately regulated, identifying their individual effects on stroke has hitherto been difficult. These results show that Gal-3 is necessary for angiogenesis in stroke in a VEGF-dependant manner, but suggest that angiogenesis may be dispensable for post-stroke endogenous repair, therefore drawing into question the clinical utility of augmenting angiogenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurobiology of Disease - Volume 63, March 2014, Pages 155-164
Journal: Neurobiology of Disease - Volume 63, March 2014, Pages 155-164
نویسندگان
Christopher C. Young, Osama Al-Dalahmah, Nicola J. Lewis, Keith J. Brooks, Micaela M. Jenkins, Françoise Poirier, Alastair M. Buchan, Francis G. Szele,