کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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602505 | 879980 | 2007 | 5 صفحه PDF | دانلود رایگان |
In this study, we determined the protective effect of total flavonoids from Spirodela polyrrhiza (L.) Schleid (STF), which is a kind of traditional Chinese medicine, on human umbilical vein endothelial cells (ECV-304) damage induced by hydrogen peroxide (H2O2). Treated with 1 mmol/L H2O2 for 1 h, the viability of ECV-304 cells markedly decreased. However, pretreatment with 10–50 μg/mL STF resulted in a significant recovery. The survival rate of ECV-304 increased from 21.98% (only treated with 1 mmol/L H2O2) to 64.74% (pretreated with 50 μg/mL STF), which accompanied with the amounts of malondialdenhyde (MDA) decreasing from 1.6883 nmol/L to 0.9628 nmol/L. Furthermore, compared with control group, the 50 μmol/L STF pretreatment enhanced the total antioxidant capacity (T-AOC) by 4.49 times, increased the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) by 85.12%, 158.94% and 94.5%,respectively, and increased the content of nitric oxide (NO) by 116.55%.Taken together, STF protect ECV-304 cells against H2O2 damage by enhancing the antioxidant ability and increasing NO production.
Journal: Colloids and Surfaces B: Biointerfaces - Volume 60, Issue 1, 15 October 2007, Pages 36–40