کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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607993 | 880565 | 2012 | 6 صفحه PDF | دانلود رایگان |
Biomaterials capable of suppressing microbial infection are of clear importance in various health care applications, e.g. implantable devices. In this study, we investigate the antimicrobial activity of single-walled carbon nanotubes (SWNT) layer-by-layer (LbL) assembled with the polyelectrolytes poly(L-lysine) (PLL) and poly(L-glutamic acid) (PGA). SWNT dispersion in aqueous solution is achieved through the biocompatible nonionic surfactant polyoxyethylene(20) sorbitan monolaurate (Tween 20), and the amphiphilic polymer phospholipid-poly(ethylene glycol) (PL-PEG). Absorbance spectroscopy and transmission electron microscopy (TEM) show SWNT with either Tween 20 or PL-PEG in aqueous solution to be well dispersed, at about the level of SWNT in chloroform. Quartz crystal microgravimetry with dissipation (QCMD) measurements show both SWNT-Tween and SWNT-PL-PEG to LbL assemble with PLL and PGA into multilayer films, with the PL-PEG system yielding the greater final SWNT content. Escherichia coli and Staphylococcus epidermidis inactivation rates are significantly higher (up to 90%) upon 24 h incubation with SWNT containing films, compared to control films (ca. 20%). This study demonstrates the potential usefulness of SWNT/PLL/PGA thin films as antimicrobial biomaterials.
Figure optionsDownload high-quality image (319 K)Download as PowerPoint slideHighlights
► First to employ SWNT as the primary antimicrobial agent within an LbL assembled film.
► Improved aqueous dispersibility of SWNT via amphiphilic molecules: Tween & PL-PEG.
► Quantified adsorbed mass of SWNT and polymers in LBL assembly via QCMD.
► Inactivated ∼90% of bacteria (gram positive and negative) upon 24 h of contact.
Journal: Journal of Colloid and Interface Science - Volume 388, Issue 1, 15 December 2012, Pages 268–273