کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6122072 | 1219197 | 2016 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Application of water-soluble nanofilters for collection of airborne Mycobacterium tuberculosis DNA in hospital wards
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروبیولوژی و بیوتکنولوژی کاربردی
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چکیده انگلیسی
A simple inexpensive technique for collection of airborne biomarkers of nosocomial infections is described. Biomarkers were collected on water-soluble electrospun nanofilters attached to a household vacuum cleaner from 6-10Â m3 of air in 10-15Â min within several wards of a tuberculosis clinic. Filters were then dissolved in water and tested for the presence of the IS6110 and regX3 genes of Mycobacterium tuberculosis (MTB) using real-time polymerase chain reaction. It was demonstrated that trace amounts of airborne MTB DNA (>3Â gene copies/m3) were always present in the air and on the surfaces in the wards occupied with tuberculosis patients having positive smear tests.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Hospital Infection - Volume 93, Issue 1, May 2016, Pages 100-104
Journal: Journal of Hospital Infection - Volume 93, Issue 1, May 2016, Pages 100-104
نویسندگان
M.A. Vladimirsky, L.K. Shipina, E.S. Makeeva, Y.S. Alyapkina, A.Y. Mikheev, V.N. Morozov,