A real-time PCR approach based on SPF10 primers and the INNO-LiPA HPV Genotyping Extra assay for the detection and typing of human papillomavirus

► Simultaneous amplification and detection of HPV target by a SPF10 real-time PCR. ► Reduction of workload and cost by avoiding LiPA step for HPV-negative samples. ► The SPF10 real-time PCR is very sensitive. ► The SPF10 real-time PCR generates amplicons, which are compatible with the LiPA.