Anti-bacterial and anti-biofilm activity of probiotic bacteria against oral pathogens

- Three lactic acid bacteria (LAB), isolated from barley, traditional dried meat and fermented olive were characterized.
- The selected LAB were γ-hemolytic, resist to low pH, bile salt, pepsin and pancreatin.
- A significant inhibitory effect of P. pentosaceus FB2 and L. brevis FF2 against S. salivarius B468 was notice.
- The selected strains were able to inhibit biofilm formation of B. cereus and S. salivarius B468.

In this study, three lactic acid bacteria (LAB), isolated from barley, traditional dried meat and fermented olive were characterized and tested for their anti-bacterial and anti-biofilm activities against oral bacteria.Our results revealed that the tested LAB were γ-hemolytic and were susceptible to four antibiotics. All the strains were resistant to low pH, bile salt, pepsin and pancreatin. Furthermore, FB2 displayed a high aut-oaggregative phenotype (99.54%) while FF2 exhibited the best co-aggregation rate. Concerning the microbial adhesion to solvent, FB2 was the most hydrophobic strain (data obtained with chloroform and n-hexadecane). In addition Pediococcus pentosaceus FB2 and Lactobacillus brevis FF2 displayed a significant inhibitory effect against Streptococcus salivarius B468 (MIC = 10%). Moreover the selected strains were able to inhibit biofilm formation of Bacillus cereus ATCC14579 (MBIC50 = 28.16%) and S. salivarius B468 (MBIC50 = 42.28%).The selected LAB could be considered as candidate probiotics for further application in functional food and mainly in the prevention of oral diseases.