کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6272036 | 1614776 | 2015 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Downregulation of survivin regulates adult hippocampal neurogenesis and apoptosis, and inhibits spatial learning and memory following traumatic brain injury
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کلمات کلیدی
PCNAPBSshRNAsNSCSGZDAPIPFAIAPDcxMWMLFP4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینول5′-bromo-2′-deoxyuridine - 5'-bromo-2'-deoxyuridineNPCs - NPC هاProliferating Cell Nuclear Antigen - آنتیژن هسته ای تکثیر سلولیBrdU - بروموداکسی اوریدینanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of variancedoublecortin - دوچرخهneural stem/progenitor cell - سلول های بنیادی عصبی / پرونوتیکNeural precursor cells - سلول های پیشگیر عصبیdentate gyrus - شکنج دندانه دارescape latency - فرار از تأخیرMorris water maze - ماز آب آب موریسPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریsubgranular zone - منطقه غده گرانولیinhibitor of apoptosis protein - مهار کننده پروتئین آپوپتوزیسparaformaldehyde - پارافرمالدهیدLateral fluid percussion - کوپه مفاصل جانبی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Downregulation of survivin regulates adult hippocampal neurogenesis and apoptosis, and inhibits spatial learning and memory following traumatic brain injury Downregulation of survivin regulates adult hippocampal neurogenesis and apoptosis, and inhibits spatial learning and memory following traumatic brain injury](/preview/png/6272036.png)
چکیده انگلیسی
Survivin, a unique member of the inhibitor of the apoptosis protein (IAP) family, has been suggested to play a crucial role in promoting the cell cycle and mediates mitosis during embryonic development. However, the role of survivin following traumatic brain injury (TBI) in adult neurogenesis and apoptosis in the mouse dentate gyrus (DG) remains only partially understood. We adopted adenovirus-mediated RNA interference (RNAi) as a means of suppressing the expression of survivin and observed its effects on adult regeneration and neurological function in mice after brain injury. The mice were subjected to TBI, and the ipsilateral hippocampus was then examined using reverse transcription polymerase chain reaction (RT-PCR) and Western blotting analyses. Brain slices were stained for 5â²-bromo-2â²-deoxyuridine (BrdU) and doublecortin (DCX). Our data showed that survivin knockdown inhibits the proliferation and differentiation of neural precursor cells (NPCs) in the DG of the hippocampus soon after TBI. Furthermore, downregulation of survivin results in a significant increase in programmed cell death in the DG, as assessed using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and 4â²,6-diamidino-2-phenylindole (DAPI) double staining. The Morris water maze (MWM) test was adopted to evaluate neurological function, which confirmed that knockdown of survivin worsened the memory capacity that was already compromised following TBI. Survivin in adult mice brains after TBI can be successfully down-regulated by RNAi, which inhibited adult hippocampal neurogenesis, promoted apoptotic cell death, and resulted in a negative role in the recovery of dysfunction following injury.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 300, 6 August 2015, Pages 219-228
Journal: Neuroscience - Volume 300, 6 August 2015, Pages 219-228
نویسندگان
Z. Zhang, H. Wang, Z. Jin, X. Cai, N. Gao, X. Cui, P. Liu, J. Zhang, S. Yang, X. Yang,