کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6273367 | 1614796 | 2014 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Motoneuron regeneration accuracy and recovery of gait after femoral nerve injuries in rats
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کلمات کلیدی
MDCPMRmotoneuronsMNsp.o.FBAICCSLRFpsBehavioral test - آزمون رفتاریstandard deviation - انحراف معیارPeripheral nerve regeneration - بازسازی عصب محیطیPost-operative - بعد از عملANOVA - تحلیل واریانس Analysis of varianceone-way analysis of variance - تحلیل واریانس یک راههMinimal detectable change - تغییر قابل ملاحظه حداقلstandard error of mean - خطای استاندارد میانگینPreferential motor reinnervation - رانندگی موتور ترجیحیrecovery index - شاخص بازیابیCoefficient of Variation - ضریب تغییرCoefficient of correlation - ضریب همبستگیInterclass Correlation Coefficient - ضریب همبستگی بین کلاسیکNumber - عددframes per second - فریم بر ثانیهSEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
The rat femoral nerve is a valuable model allowing studies on specificity of motor axon regeneration. Despite common use of this model, the functional consequences of femoral nerve lesions and their relationship to precision of axonal regeneration have not been evaluated. Here we assessed gait recovery after femoral nerve injuries of varying severity in adult female Wistar rats using a video-based approach, single-frame motion analysis (SFMA). After nerve crush, recovery was complete at 4Â weeks after injury (99% of maximum 100% as estimated by a recovery index). Functional restoration after nerve section/suture was much slower and incomplete (84%) even 20Â weeks post-surgery. A 5-mm gap between the distal and proximal nerve stumps additionally delayed recovery and worsened the outcome (68% recovery). As assessed by retrograde labeling in the same rats at 20Â weeks after injury, the anatomical outcome was also dependent on lesion severity. After nerve crush, 97% of the femoral motoneurons (MNs) had axons correctly projecting only into the distal quadriceps branch of the femoral nerve. The percentage of correctly projecting MNs was only 55% and 15% after nerve suture and gap repair, respectively. As indicated by regression analyses, better functional recovery was associated with higher numbers of correctly projecting MNs and, unexpectedly, lower numbers of MNs projecting to both muscle and skin. The data show that type of nerve injury and repair profoundly influence selectivity of motor reinnervation and, in parallel, functional outcome. The results also suggest that MNs' projection patterns may influence their contribution to muscle performance. In addition to the experiments described above, we performed repeated measurements and statistical analyses to validate the SFMA. The results revealed high accuracy and reproducibility of the SFMA measurements.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 280, 7 November 2014, Pages 73-87
Journal: Neuroscience - Volume 280, 7 November 2014, Pages 73-87
نویسندگان
M. Kruspe, H. Thieme, O. Guntinas-Lichius, A. Irintchev,