Development of real-time PCR methods to quantify patulin-producing molds in food products

► Development of RTi-PCR tests based on idh gene to quantify patulin-producing molds. ► A high linear correlation between idh gene copy number and Ct values is found. ► RTi-PCR SYBR Green and TaqMan probe developed showed high sensitivity in foods. ► These methods allow an accurate quantification of patulin fungal growth in foods. ► RTi-PCR developed should be used to monitor patulin-producing mold in foods.