Identification and characterization by PCR-RFLP analysis of the genetic variation for the Glu-A1x and Glu-B1x genes in rivet wheat (Triticum turgidum L. ssp. turgidum)

► The Glu-A1x and Glu-B1x genes of rivet wheat were analysed by PCR and endonuclease digestion. ► The restriction patterns were clear enough to differentiate between alleles. ► The PCR-RFLP technique allows identifying allelic variants not seen with SDS-PAGE or PCR techniques.