Development of monoclonal antibody-based competitive immunoassays for the detection of picoxystrobin in cereal and oilseed flours

► Regioisomeric haptens were used to generate novel monoclonal antibodies to picoxystrobin. ► Direct and indirect competitive immunoassays using homologous and heterologous antigens were optimized. ► Both assays were specific and showed a limit of detection around 0.1 μg/L. ► The new ELISAs were successfully applied to the determination of picoxystrobin in cereal flours. ► The described immunoreagents are promising tools for different immunoanalytical platforms.