In vitro mutation breeding of Paphiopedilum by ionization radiation

► The optimum condition for culturing the PLBs, shoot buds and in vitro plantlets of endemic and endangered rare Paphiopedilum of Vietnam and the three Indochinese countries. ► The LD50 values of PLBs, shoot buds and plantlets of Paphiopedilum. ► Generation of in vitro variations of Paphiopedilum by ionization irradiation. ► RAPD analysis of mutant lines.