کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
641082 | 1456984 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Homogenate-assisted negative-pressure cavitation extraction for determination of organic acids and flavonoids in honeysuckle (Lonicera japonica Thunb.) by LC-MS/MS
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تصفیه و جداسازی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In the present study, a homogenate-assisted negative-pressure cavitation extraction (H-NPCE) is developed for the extraction and determination of the main organic acids and flavonoids in honeysuckle by LC-MS/MS. The influential parameters of the H-NPCE procedure were optimized, and the optimal conditions were as follows: homogenate time 45 s, negative pressure â0.07 MPa, ethanol concentration 70%, liquid/solid ratio 20:1 mL/g, NPCE time 20 min and 3 extraction cycles. Furthermore, a rapid LC-MS/MS analysis method was established and validated, and it exhibited satisfactory linearity (R2 > 0.996), precision (RSD ⩽ 4.87%) and recovery (96.94-104.32%). H-NPCE followed by LC-MS/MS was successfully applied for the extraction and quantification of ten organic acids and flavonoids in honeysuckle. This work provided a fast and effective method for the sample preparation and determination of active components from plants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Separation and Purification Technology - Volume 135, 15 October 2014, Pages 80-87
Journal: Separation and Purification Technology - Volume 135, 15 October 2014, Pages 80-87
نویسندگان
Xi-Qing Wang, Fu-Yao Wei, Zuo-Fu Wei, Lin Zhang, Meng Luo, Yu-Hang Zhang, Yuan-Gang Zu, Yu-Jie Fu,