Biosorption of aluminum through the use of non-viable biomass of Pseudomonas putida

- Non-living biomass of Pseudomonas putida for the removal of Al3+.
- Phosphatidylcholine of the cell membranes as binding site of Al3+.
- The efficiency of adsorption of Al3+ is depending on the number of binding sites.

Living and non-living biomass of Pseudomonas putida A (ATCC 12633) was used as biosorbent for the removing of Al3+ from aqueous solutions. The process was stable with time, efficient at pH 4.3 and between 15 °C and 42 °C. Two isotherms models were applied to describe the interaction between the biosorbent and Al3+. Non-living biomass of P. putida A (ATCC 12633) was found to be the most efficient at adsorbing Al3+ with a maximum sorption capacity of 0.55 mg Al3+/gr adsorbent and with 36 × 105 binding sites of Al3+/microorganisms. Infrared spectroscopy analysis shows that the biosorbent present some vibrational band of functional groups that change in presence of Al3+: hydroxyl, carboxyl and phosphate. Considering that Al3+ binds to the phosphate group of phosphatidylcholine, non-viable biomass of P. putida PB01 (mutant lacking phosphatidylcholine) was used. Aluminum adsorption of the parental strain was 30 times higher than values registered in P. putida PB01 (36 × 105 sites/microorganism vs 1.2 × 105 sites/microorganism, respectively). This result evidenced that the absence of phosphatidylcholine significantly affected the availability of the binding sites and consequently the efficiency of the biomass to adsorb Al3+.