کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6452626 1418335 2017 15 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The importance of sourcing enzymes from non-conventional fungi for metabolic engineering and biomass breakdown
ترجمه فارسی عنوان
اهمیت استخراج آنزیم از قارچ های غیر متعارف برای مهندسی متابولیک و شکست زیست توده
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی


- A wealth of enzymes in industrial use are sourced from fungi.
- Only a few fungal clades have been explored for metabolic engineering applications.
- Novel biomass-degrading functions are likely to be found in the basal clades.
- Anaerobic fungi have the highest diversity of biomass-degrading enzymes across fungi.
- Modern biotechnology promises to unlock the potential of non-model fungi.

A wealth of fungal enzymes has been identified from nature, which continue to drive strain engineering and bioprocessing for a range of industries. However, while a number of clades have been investigated, the vast majority of the fungal kingdom remains unexplored for industrial applications. Here, we discuss selected classes of fungal enzymes that are currently in biotechnological use, and explore more basal, non-conventional fungi and their underexploited biomass-degrading mechanisms as promising agents in the transition towards a bio-based society. Of special interest are anaerobic fungi like the Neocallimastigomycota, which were recently found to harbor the largest diversity of biomass-degrading enzymes among the fungal kingdom. Enzymes sourced from these basal fungi have been used to metabolically engineer substrate utilization in yeast, and may offer new paths to lignin breakdown and tunneled biocatalysis. We also contrast classic enzymology approaches with emerging 'omics'-based tools to decipher function within novel fungal isolates and identify new promising enzymes. Recent developments in genome editing are expected to accelerate discovery and metabolic engineering within these systems, yet are still limited by a lack of high-resolution genomes, gene regulatory regions, and even appropriate culture conditions. Finally, we present new opportunities to harness the biomass-degrading potential of undercharacterized fungi via heterologous expression and engineered microbial consortia.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Metabolic Engineering - Volume 44, November 2017, Pages 45-59
نویسندگان
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