کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6481367 1398100 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Preparation of chitosan-based multifunctional nanocarriers overcoming multiple barriers for oral delivery of insulin
ترجمه فارسی عنوان
آماده سازی نانوذرات چند منظوره مبتنی بر کیتوزان برای غلبه بر موانع متعدد برای تحویل دهان انسولین
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
چکیده انگلیسی


- Chitosan-based multifunctional nanocarriers for oral delivery of insulin were prepared.
- The resultant nanocarriers exhibited low cytotoxicity against HT-29 cells.
- The loaded insulin exhibited excellent stability against pepsin and pancreatin.
- The hypoglycemic effect for oral delivery of insulin was studied in vivo.
- The findings provide a new way in diabetes treatment via oral ingestion.

To overcome multiple barriers for oral delivery of insulin, the chitosan-based multifunctional nanocarriers modified by L-valine (LV, used as a target ligand to facilitate the absorption of the small intestine) and phenylboronic acid (PBA, used as a glucose-responsive unit) have been designed and evaluated in this study. The resultant nanocarriers exhibited low cytotoxicity against HT-29 cells and excellent stability against protein solution. The insulin release behaviors were evaluated triggered by pH and glucose in vitro. The chemical stability of loaded insulin against digestive enzyme were established in presence of simulated gastric fluid (SGF) containing pepsin and simulated intestinal fluid (SIF) containing pancreatin, respectively. The uptake behavior of HT-29 cells was evaluated by confocal laser scanning microscope. After oral administration to the diabetic rats, an effective hypoglycemic effect was obtained compared with subcutaneous injection of insulin. This work suggests that L-valine modified chitosan-based multifunctional nanocarriers may be a promising drug delivery carrier for oral administration of insulin.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 70, Part 1, 1 January 2017, Pages 278-286
نویسندگان
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