کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6481400 | 1398100 | 2017 | 14 صفحه PDF | دانلود رایگان |
- PAMAM-functionalized GO was prepared and used to co-deliver DOX and MMP-9 shRNA plasmid.
- It showed an effective delivery of DOX and MMP-9 to MCF-7 cells.
- The co-loaded PAMAM-GO suggested a potential application in cancer therapy.
It is a promising way to treat the multi drug resistance (MDR) of tumor cells in both of drug and gene methods. A polyamidoamne dendrimer functionalized graphene oxide (GO-PAMAM) was designed, which could load doxorubicin (DOX) and MMP-9 shRNA plasmid at the same time in order to achieve effective treatment to breast cancer. GO-PAMAM has a high loading capacity to DOX and pH-controlled DOX release. Besides, it has efficient gene transfer ability, the transfection efficiency is significantly better than PEI-25k in the presence of serum, and it can significantly inhibit the expression of MMP-9 protein in MCF-7 cells. The effect of DOX and MMP-9 shRNA plasmid co-delivery was more significant than that of the single drug. Moreover, GO-PAMAM exhibited lower cytotoxicity compared to PEI-25k in CCK-8 assays, and also showed a good biocompatibility in vivo. Therefore, GO-PAMAM will have broad prospects for drug and gene co-delivery.
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Journal: Materials Science and Engineering: C - Volume 70, Part 1, 1 January 2017, Pages 572-585