کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6490894 | 43383 | 2015 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sequential/parallel production of potential Malaria vaccines - A direct way from single batch to quasi-continuous integrated production
ترجمه فارسی عنوان
واکسن های بالقوه مالاریا به صورت متوالی / موازی - یک راه مستقیم از یک سری به تولید مجتمع نیمه مداوم
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
An intensification of pharmaceutical protein production processes can be achieved by the integration of unit operations and application of recurring sequences of all biochemical process steps. Within optimization procedures each individual step as well as the overall process has to be in the focus of scientific interest. This paper includes a description of the development of a fully automated production plant, starting with a two step upstream followed by a four step downstream line, including cell clarification, broth cleaning with microfiltration, product concentration with ultrafiltration and purification with column chromatography. Recursive production strategies are developed where a cell breeding, the protein production and the whole downstream is operated in series but also in parallel, each main operation shifted by one day. The quality and reproducibility of the recursive protein expression is monitored on-line by Golden Batch and this is controlled by Model Predictive Multivariate Control (MPMC). As a demonstration process the production of potential Malaria vaccines with Pichia pastoris is under investigation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 213, 10 November 2015, Pages 83-96
Journal: Journal of Biotechnology - Volume 213, 10 November 2015, Pages 83-96
نویسندگان
Reiner Luttmann, Sven-Oliver Borchert, Christian Mueller, Kai Loegering, Florian Aupert, Stephan Weyand, Christian Kober, Bart Faber, Gesine Cornelissen,