Immunoassay of C-reactive protein by hot electron-induced electrochemiluminescence at polystyrene-carbon black composite electrodes

C-reactive protein was finally determined in a heterogeneous sandwich-assay by using the present composite electrodes as a solid support material for the capture antibodies. The detection antibody was labeled with a commercially available Tb(II) chelate. Calibration curve for the determination of C-reactive protein was linear over two orders of magnitude and the detection limit was well below the clinical reference value, approx. 1 μg l−1. Disposable polystyrene-carbon black composite electrodes are superior alternatives for e.g. silicon-based electrodes due to the low cost, easier manufacturing process and a very good performance e.g. in immunoassays.