High frequency direct organogenesis and evaluation of genetic stability for in vitro regenerated Pongamia pinnata, a valuable biodiesel plant

► Analyzed the in vitro propagation and genetic clonality in plus tree of Pongamia. ► Compared the response of epicotyl and nodal explants on MS and WP media. ► WP is more effective than MS media for obtaining a large number of plantlets. ► RAPD and ISSR are effective for genetic stability in micropropagated Pongamia. ► The study paves future research with regard to breeding aspect in biodiesel crop.