The influence of presaccharification, fermentation temperature and yeast strain on ethanol production from sugarcane bagasse

Ethanol can be produced from cellulosic biomass in a process known as simultaneous saccharification and fermentation (SSF). The presence of yeast together with the cellulolytic enzyme complex reduces the accumulation of sugars within the reactor, increasing the ethanol yield and saccharification rate. This paper reports the isolation of Saccharomyces cerevisiae LBM-1, a strain capable of growth at 42 °C. In addition, S. cerevisiae LBM-1 and Kluyveromyces marxianus UFV-3 were able to ferment sugar cane bagasse in SSF processes at 37 and 42 °C. Higher ethanol yields were observed when fermentation was initiated after presaccharification at 50 °C than at 37 or 42 °C. Furthermore, the volumetric productivity of fermentation increased with presaccharification time, from 0.43 g/L/h at 0 h to 1.79 g/L/h after 72 h of presaccharification. The results suggest that the use of thermotolerant yeasts and a presaccharification stage are key to increasing yields in this process.

► We isolated of the S. cerevisiae LBM-1 strain, which is capable of growth at 42 °C.
► We compared the capacity of different yeast strains to ferment sugar cane bagasse.
► Presaccharification stage is key to increasing ethanol yields.
► Use of thermotolerant yeasts increase the yield of the SSF process.