Oleuropein extraction using microfluidic system

• Microfluidic device used for continuous extraction of oleuropein from ethyl acetate.
• Influence of pH, temperature, flow rate ratio and residence time were investigated.
• Extraction yield of 68.7% obtained under the optimum conditions with RSD of 1.2%.
• The advantage of oleuropein extraction in microchannel over batch process was proved.

In the present study, microfluidic devices have been used for extraction of oleuropein from ethyl acetate into aqueous phase. The effects of aqueous phase pH, temperature, flow rate ratio and residence time were investigated and optimized. Deionized water as extractant phase, ambient temperature, flow rate ratio of 1 and residence time of 0.1293 min were chosen as the best conditions. The content of oleuropein was determined by using high-performance liquid chromatography (HPLC). The extraction yield of 68.7% was obtained under the optimum conditions with relative standard deviation of 1.2%. The experimental results of microchannel extraction were compared with the batch process. The results revealed that the extraction yield using microchannel extractor device was more than that of conventional batch process. The results illustrate that the proposed technique has some advantages comparing with other methods including; simplicity of operation, cost effective and environmentally friendly.

A schematic diagram of the experimental setup.Figure optionsDownload as PowerPoint slide