کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6972155 1453098 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Extracellular polymeric substances from copper-tolerance Sinorhizobium meliloti immobilize Cu2+
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بهداشت و امنیت شیمی
پیش نمایش صفحه اول مقاله
Extracellular polymeric substances from copper-tolerance Sinorhizobium meliloti immobilize Cu2+
چکیده انگلیسی
The copper tolerance gene of wild-type heavy metal-tolerance Sinorhizobium meliloti CCNWSX0020 was mutated by transposon Tn5-a. The mutant was sensitive up to 1.4 mM Cu2+. Production, components, surface morphology, and functional groups of extracellular polymeric substances (EPS) of the wild-type strains were compared with sensitive mutant in immobilization of Cu2+. EPS produced by S. meliloti CCNWSX0020 restricts uptake of Cu2+. The cell wall EPS were categorized based on the compactness and fastness: soluble EPS (S-EPS), loosely bound EPS (LB-EPS), and tightly bound EPS (TB-EPS). LB-EPS played a more important role than S-EPS and TB-EPS in Cu2+ immobilization. Scanning electron microscopy (SEM) analysis LB-EPS had rough surface and many honeycomb pores, making them conducive to copper entry; therefore, they may play a role as a microbial protective barrier. Fourier transform-infrared (FT-IR) analysis further confirm that proteins and carbohydrates were the main extracellular compounds which had functional groups such as carboxyl (COOH), hydroxyl (OH), and amide (NH), primarily involved in metal ion binding.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Hazardous Materials - Volume 261, 15 October 2013, Pages 614-620
نویسندگان
, , , , , , , ,