A perspective on the characterization of colloids and macromolecules using asymmetrical flow field-flow fractionation

- AF4 aids the characterization of the physicochemical properties, such as shape and aggregation state of colloidal materials.
- AF4 is a method of choice for analyzing protein aggregates in therapeutics as recommended by the FDA.
- Aggregated structures of macromolecules are separated and characterized by AF4 providing insight into their functionality.