Binding study of novel anti-diabetic pyrimidine fused heterocycles to β-lactoglobulin as a carrier protein

- The novel PFH derivatives (L1-L4) efficiently interacted with β-LG due to a ground state complex formation.
- The ligands and β-LG form a single complex (1:1) and L4 proved to have highest affinity towards β-LG.
- The nature of interactions was proved to be hydrophobic, hydrogen bonding and vander Waals forces.
- No structural alteration was induced in the secondary structures of β-LG due to the complexation with ligands.