کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
70433 48829 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparison of polypyrrole-based xanthine oxidase amperometric and potentiometric biosensors for hypoxanthine
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی کاتالیزور
پیش نمایش صفحه اول مقاله
Comparison of polypyrrole-based xanthine oxidase amperometric and potentiometric biosensors for hypoxanthine
چکیده انگلیسی

The preparation and performance of xanthine oxidase electrochemical biosensor developed for the determination of hypoxanthine is reported. Xanthine oxidase (XOD) and a mediator potassium ferrocyanide (K4Fe(CN) were entrapped into a polypyrrole (PPy) film during galvanostatic film formation. The optimum conditions for the formation of PPy-XOD-Fe(CN)64− film include 0.4 M pyrrole, 6.2 U/mL XOD, 40 mM K4Fe(CN)6, polymerisation period of 200 s, and an applied current density of 0.5 mA/cm2. Two modes of detection, the potentiometric and amperometric modes, were investigated. The optimum potential for the amperometric biosensing of hypoxanthine was 800 mV vs. Ag/AgCl (3 M KCl) in 0.05 M phosphate buffer. A comparison of the sensitivities for the amperometric and potentiometric modes revealed that potentiometric detection was more sensitive and amperometric detection gave a wider linear concentration range. The PPy-XOD biosensor was successfully used for the determination of fish freshness.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 66, Issues 3–4, October 2010, Pages 270–275
نویسندگان
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