کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7077744 | 1459979 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Relative extents of activity loss between enzyme-substrate interactions and combined environmental mechanisms
ترجمه فارسی عنوان
مقادیر نسبی از دست دادن فعالیت بین واکنشهای آنزیم-سوبسترا و مکانیسم های محیطی ترکیب شده
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
چکیده انگلیسی
Enzymatic hydrolysis of biomass undergoes a significant decrease in rate, which is often attributed to activity loss of enzyme during the incubation. Activity loss due to both interaction with substrate (for example inactivation of adsorbed enzyme) and all combined environmental mechanisms in a substrate free buffer solution were compared in this study. Enzyme-substrate interactions contributed more towards the overall activity loss than did the combined environmental sources as evidenced from three independent metrics. (1) Relative extents of inactivation were higher for enzyme-substrate interactions than for environmental mechanisms. (2) Apparent half-lives (1.37-11.01Â h) following interaction with substrate were relatively small compared to environmental inactivation, which was 21.5Â h. (3) The inactivation rate constant for enzyme-substrate interactions (0.56Â hâ1) was 46 times higher than that of environmental inactivation (0.0123Â hâ1). These results suggest enzyme-substrate interaction is the main cause of cellulase activity loss and contributes significantly to the slow rate of hydrolysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 164, July 2014, Pages 143-148
Journal: Bioresource Technology - Volume 164, July 2014, Pages 143-148
نویسندگان
Zhuoliang Ye, Kristen M. Hatfield, R. Eric Berson,