Pretreatment based on two-step steam explosion combined with an intermediate separation of fiber cells-Optimization of fermentation of corn straw hydrolysates

► Two-step steam explosion with ISFC process was established. ► Inhibitor content was reduced by 33% by this process. ► Fibrous tissues were separated from parenchyma by this process. ► Enzymatic hydrolysis and fermentation products conversion were increased by this process.