Inhibition of xyloglucanase from an alkalothermophilic Thermomonospora sp. by a peptidic aspartic protease inhibitor from Penicillium sp. VM24

► A bifunctional inhibitor against xyloglucanase and aspartic protease has been isolated. ► Kinetic interactions of xyloglucanase and inhibitor revealed non-competitive inhibition. ► Inactivation of xyloglucanase is due to the irreversible denaturation of the enzyme. ► The inhibitor is potential as a biocontrol agent for plant protection against phytopathogenic fungi.