کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7138773 | 1462017 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Chip-based biosensor for the detection of low adrenaline concentrations to support adrenal venous sampling
ترجمه فارسی عنوان
برای اندازه گیری غلظت آدرنالین کم برای حمایت از نمونه برداری وریدی آدرنال، بر اساس سنسورهای مبتنی بر تراشه طراحی شده است
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A chip-based amperometric biosensor referring on using the bioelectrocatalytical amplification principle for the detection of low adrenaline concentrations is presented. The adrenaline biosensor has been prepared by modification of a platinum thin-film electrode with an enzyme membrane containing the pyrroloquinoline quinone-dependent glucose dehydrogenase and glutaraldehyde. Measuring conditions such as temperature, pH value, and glucose concentration have been optimized to achieve a high sensitivity and a low detection limit of about 1â¯nM adrenaline measured in phosphate buffer at neutral pH value. The response of the biosensor to different catecholamines has also been proven. Long-term stability of the adrenaline biosensor has been studied over 10â¯days. In addition, the biosensor has been successfully applied for adrenaline detection in human blood plasma for future biomedical applications. Furthermore, preliminary experiments have been carried to detect the adrenaline-concentration difference measured in peripheral blood and adrenal venous blood, representing the adrenal vein sampling procedure of a physician.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 272, 1 November 2018, Pages 21-27
Journal: Sensors and Actuators B: Chemical - Volume 272, 1 November 2018, Pages 21-27
نویسندگان
Denise Molinnus, Gabriel Hardt, Larissa Käver, Holger S. Willenberg, Jens-Christian Kröger, Arshak Poghossian, Michael Keusgen, Michael J. Schöning,