کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7139603 | 1462022 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A ratiometric electrochemical deoxyribonucleic acid sensing strategy based on self-signal of highly stable reduced graphene oxide-flavin mononucleotide aqueous dispersion modified nanointerface
ترجمه فارسی عنوان
یک استراتژی سنجش سنجی دز اکسید ریبونوکلئیک اسید الکتروشیمیایی ریاضی براساس سیگنال خودتنظیم کاهش ناگهانی تغییر شکل پراکندگی گرافن اکسید فلاوین مونونوکلئوتید گرافن
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A label-free ratiometric electrochemical DNA sensing strategy was established by simultaneously adopting the self-redox signal of electrode interface and signal of electrochemical indicator as ratiometric signals together. The sodium salt of flavin mononucleotide (FMNs)/reduced graphene oxide(rGO) nanocomposite was prepared by simple ultrasonication method. The obtained FMNs/rGO possesses high water dispersion and good electrochemical redox signal. FMNs/rGO was further applied as the electrode modification material to immobilize DNA probe and provide the self-redox signal for DNA detection, while the electrochemical indicator Meldola's Blue (MDB) was served as another detection signal. With the increase of target DNA concentration, the self-redox signal of the FMNs/rGO would decrease due to the formation of more DNA duplex, while more MDB would combine with the DNA duplex and resulted in higher signal of MDB. By measuring the ratios of the two signals, this novel ratiometric strategy effectively improved the reproducibility and reliability while avoided using expensive and complicated redox labels on DNA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 267, 15 August 2018, Pages 519-524
Journal: Sensors and Actuators B: Chemical - Volume 267, 15 August 2018, Pages 519-524
نویسندگان
Tao Yang, Renzhong Yu, Sumin Liu, Zhiwei Qiu, Shizhong Luo, Weihua Li, Kui Jiao,