کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7140589 1462026 2018 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Magnetic-assisted aptamer-based fluorescent assay for allergen detection in food matrix
ترجمه فارسی عنوان
آزمایش فلورسنت مبتنی بر آپتامر با استفاده از مغناطیسی برای تشخیص آلرژن در ماتریکس مواد غذایی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Herein, a facile and versatile label-free aptamer-based fluorescent assay for simple and sensitive detection of food allergen in food matrix is developed based on the functionalized magnetic nanoparticles (MNPs) as separation carrier and commercially available OliGreen dye as signal probe. First, the aptamer hybridized with capture probe that was conjugated on the surface of MNPs to form aptamer-MNPs complexes as detection probes, which undergo conformational change upon interaction with target analytes, resulting in the release of aptamer from the surface of MNPs. So the released aptamer in the supernatant produced a significant fluorescence enhancement signal by introducing the OliGreen dye based on its ultrasensitive and specific fluorescence enhancement upon binding to ssDNA. As proof-of concept, this proposed strategy was applied to quantitative detection of tropomyosin, one of the major allergen found in shellfish. Under optimal conditions, the linear range was from 0.4 to 5 μg mL−1 (R2 = 0.996) with a low limit of detection calculated to be 77 ng mL−1. Moreover, the aptamer-based assay had remarkable selectivity and was successfully applied in the detection of tropomyosin in food matrix. Due to its simple design, easy operation and high sensitivity and selectivity, the proposed aptamer assay could expect to expand applications in various allergens detection by changing corresponding aptamers on the detection probe.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 263, 15 June 2018, Pages 43-49
نویسندگان
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