کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7229546 | 1470923 | 2018 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Glucose biosensor based on disposable electrochemical paper-based transducers fully fabricated by screen-printing
ترجمه فارسی عنوان
گاوآسیبنسور بر اساس مبدل های الکتروشیمیایی یکبار مصرف مبتنی بر کاغذ که به طور کامل توسط چاپ روی صفحه ساخته شده است
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کلمات کلیدی
دستگاه مبتنی بر کاغذ، چاپ صفحه نمایش، ساخت، الکترود، بیوسنسور گلوکز، نوشیدنی بدون الکل،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
This paper describes a new approach for the massive production of electrochemical paper-based analytical devices (ePADs). These devices are fully fabricated by screen-printing technology and consist of a lineal microfluidic channel delimited by hydrophobic walls (patterned with diluted ultraviolet screen-printing ink in chromatographic paper grade 4) and a three-electrode system (printed with carbon and/or Ag/AgCl conductive inks). The printing process was characterised and optimized for pattern each layer with only one squeeze sweep. These ePADs were used as transducers to develop a glucose biosensor. Ionic strength/pH buffering salts, electrochemical mediator (ferricyanide) and enzyme (glucose dehydrogenase FAD-dependent) were separately stored along the microfluidic channel in order to be successively dissolved and mixed after the sample dropping at the entrance. The analyses required only 10â¯Âµl and the biosensors showed good reproducibility (RSDâ¯=â¯6.2%, nâ¯=â¯10) and sensitivity (0.426â¯C/Mâ¯cm2), wide linear range (0.5-50â¯mM; r2 =â¯0.999) and low limit of detection (0.33â¯mM). Furthermore, the new biosensor was applied for glucose determination in five commercial soft-drinks without any sample treatment before the analysis. These samples were also analysed with a commercial enzymatic-kit assay. The results indicated that both methods provide accurate results.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 109, 30 June 2018, Pages 8-12
Journal: Biosensors and Bioelectronics - Volume 109, 30 June 2018, Pages 8-12
نویسندگان
P.J. Lamas-Ardisana, G. MartÃnez-Paredes, L. Añorga, H.J. Grande,