کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7233127 | 1470970 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Label-free alpha fetoprotein immunosensor established by the facile synthesis of a palladium-graphene nanocomposite
ترجمه فارسی عنوان
ایمونوسنزر آلفا فتوپروتئین آلفا بدون برچسب بدون سمیت نانوکامپوزیت پالادیم گرافن
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کلمات کلیدی
نانوذرات پالادیوم، نانوساختار گرافن، ایمونوسنسور، فتو پروتئین آلفا،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In this study, we established a sensitive label-free immunosensor by palladium-reduced graphene oxide (Pd-rGO), which was prepared by one-pot synthesis under the reduction of extra-injected CO gas. The Pd-rGO nanocomposite structure has been confirmed by TEM, SEM, X-ray diffraction, and UV-vis spectroscopy. The Pd-rGO nanocomposite exhibited excellent stability in aqueous dispersion. The Pd-rGO-based label-free electrochemical immunosensor was used for detection of the hepatocellular carcinoma (HCC) biomarker alpha fetoprotein (AFP). The immunosensor determination was based on the fact that due to the formation of antigen-antibody immunocomplex, the decreased response amperometric currents of H2O2 were directly proportional to the concentrations of AFP. The limit of detection of this immunosensor for AFP detection is 5Â pg/mL, and is linear from 0.01 to 12Â ng/mL. The proposed immunosensor has been used to determine AFP in clinical serum samples with satisfactory results. This suggests the sensor may have great potential utility in the clinic.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 61, 15 November 2014, Pages 245-250
Journal: Biosensors and Bioelectronics - Volume 61, 15 November 2014, Pages 245-250
نویسندگان
TingTing Qi, JinFeng Liao, YiSong Li, JinRong Peng, WenTing Li, BingYang Chu, He Li, YuQuan Wei, ZhiYong Qian,