کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7233279 | 1470967 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel signal-on electrochemical DNA sensor based on target catalyzed hairpin assembly strategy
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We describe a novel signal-on electrochemical DNA (E-DNA) sensing platform based on target-catalyzed hairpin assembly. The thiolated modified molecular beacon 1 (MB1) was first immobilized onto the Au electrode (GE) surface and then target DNA hybridized to the MB1, the opened MB1 assembled with the ferrocene (Fc)-labeled molecular beacon 2 to displace the target DNA, which became available for the next cycle of MB1âtarget hybridization. Moreover, Fc was confined close to the GE surface for efficient electron transfer, resulting in a current signal. Eventually, each target strand went through many cycles, resulting in numerous Fcs confining close to the GE, which leaded to the current of Fc dramatically increase. The observed signal gain was sufficient to achieve a demonstrated detection limit of 0.74Â fM, with a wide linear dynamic range from 10â15 to 10â10Â M and discriminated mismatched DNA from perfect matched target DNA with a high selectivity. Thus, the proposed E-DNA sensor would have a wide range of sensor applications because it is enzyme-free and simple to perform.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 64, 15 February 2015, Pages 177-181
Journal: Biosensors and Bioelectronics - Volume 64, 15 February 2015, Pages 177-181
نویسندگان
Yong Qian, Daoquan Tang, Lili Du, Yanzhuo Zhang, Lixian Zhang, Fenglei Gao,