کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7233595 | 1470974 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Tumor cell characterization and classification based on cellular specific membrane capacitance and cytoplasm conductivity
ترجمه فارسی عنوان
بررسی و طبقه بندی سلول های تومور بر اساس ظرفیت غشای سلولی و هدایت سیتوپلاسم
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
This paper reports a microfluidic system that enables the characterization of tumor cell electrical properties where cells were aspirated through a constriction channel (cross-section area smaller than that of biological cells) with cellular impedance profiles measured and translated to specific membrane capacitance (Cspecific membrane) and cytoplasm conductivity (Ïcytoplasm). Two batches of H1299 cells were quantified by the microfluidic platform with different constriction channel cross-section areas, recording no differences with statistical significance (p<0.001) in both Cspecific membrane (1.63±0.52 vs. 1.65±0.43 μF/cm2) and Ïcytoplasm (0.90±0.19 vs. 0.92±0.15 S/m), and thus confirming the reliability of the microfluidic platform. For paired high- and low-metastatic carcinoma strains 95D (ncell=537) and 95C cells (ncell=486), significant differences in both Cspecific membrane (2.00±0.43 vs. 1.62±0.39 μF/cm2) and Ïcytoplasm (0.88±0.46 vs. 1.25±0.35 S/m) were observed. Statistically significant difference only in Cspecific membrane (2.00±0.43 vs. 1.58±0.30 μF/cm2) was observed for 95D cells (ncell=537) and 95D CCNY-KD cells with single oncogene CCNY down regulation (ncell=479, CCNY is a membrane-associated protein). In addition, statistically significant difference only in Ïcytoplasm (0.73±0.17 vs. 1.01±0.17 S/m) was observed for A549 cells (ncell=487) and A549 CypA-KD cells with single oncogene CypA down regulation (ncell=597, CypA is a cytosolic protein). These results validated the developed microfluidic platform for Cspecific membrane and Ïcytoplasm quantification and confirmed the feasibility of using Cspecific membrane and Ïcytoplasm for tumor cell classification.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 57, 15 July 2014, Pages 245-253
Journal: Biosensors and Bioelectronics - Volume 57, 15 July 2014, Pages 245-253
نویسندگان
Y. Zhao, X.T. Zhao, D.Y. Chen, Y.N. Luo, M. Jiang, C. Wei, R. Long, W.T. Yue, J.B. Wang, J. Chen,