Measurements of optical parameters of phantom solution and bulk animal tissues in vitro at 650 nm

Optical parameters of bulk animal tissue in vitro, including absorption coefficient (μa), reduced scattering coefficient (μ′s) or scattering coefficient (μs), total attenuation coefficient (μt), anisotropy factor (g) and refractive index (n) are measured at wavelength of 650 nm. Clinical Intralipid-10% is diluted in distilled water into different concentrations to use as tissue phantoms. Four types of animal tissues in vitro are studied. The relationships among the optical parameters are analyzed systemically. For animal tissues, μa, μ′s or μs and n rely on muscle fiber orientations. μs and μt range from 10 to 20 mm−1, μa from 10−2 to 10−3 mm−1 and g from 0.95 to 0.99.