Determination of amikacin in human plasma by molecular imprinted SPR nanosensor

• We developed surface plasmon resonance sensor for detection of amikacin.
• The non-modified and AMIK-imprinted surfaces were characterized.
• The proposed method was validated according to the ICH guideline.
• The developed SPR sensor was applied to human plasma samples.

The molecular imprinting technique depends on the molecular recognition. It is polymerization method occuring around the target molecule. Thus this technique creates specific cavities in the cross-linked polymeric matrices. Amikacin (AMIK) is an important aminoglycoside (AG) antibiotic used to treat different types of bacterial infections. In this study, we prepared surface plasmon resonance (SPR) sensor for the determination of AMIK in human plasma. After the modification of gold surface of SPR chip was performed by allyl mercaptane, AMIK-imprinted poly(2-hydroxyethyl methacrylate-methacryloylamidoaspartic acid) [p(HEMA–MAAsp)] film was generated on the allyl mercaptane modified gold surface. The non-modified and imprinted surfaces were characterized by Fourier transform infrared (FTIR) spectroscopy, atomic force microscopy (AFM) and contact angle measurements. The developed method was validated according to the ICH guideline. The linearity range and the detection limit were obtained as 0.01–0.15 μg/mL and 0.0025 μg/mL (4.3 × 10−9 mol/L), respectively. The developed AMIK-imprinted nanosensor was successfully applied to the human plasma samples for the determination of AMIK.

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