کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7608056 | 1493371 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simultaneous detection of three foodborne pathogenic bacteria in food samples by microchip capillary electrophoresis in combination with polymerase chain reaction
ترجمه فارسی عنوان
تشخیص همزمان سه باکتری پاتوژن خوراکی در نمونه های غذا با استفاده از الکتروفورز مویرگی کیسه ای همراه با واکنش زنجیره ای پلیمراز
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A rapid and sensitive PCR based strategy in combination with microchip capillary electrophoresis (MCE) was employed to simultaneously detect three foodborne pathogenic bacteria. Three pairs of primers were specially designed for the amplification of target genes from Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Salmonella enterica serovar Typhimurium (S. Typhimurium). The PCR products along with standard DNA fragments were employed to optimize the separation conditions in MCE. Under optimal conditions, detectable separation of the PCR products (1.6-3.5â¯ngâ¯Î¼Lâ1) from the three foodborne pathogenic bacteria was achieved within 135â¯s. The limits of detection of the three bacteria were concluded to be as low as 45â¯CFUâ¯mLâ1 for E. coli, 62â¯CFUâ¯mLâ1 for S. aureus and 42â¯CFUâ¯mLâ1 for S. Typhimurium. The RSD of migration time was in the range of 0.5-0.8%. We conclude that MCE along with PCR holds real potential for rapid analysis and detection of nucleic acids from routine foodborne pathogenic bacteria.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1555, 22 June 2018, Pages 100-105
Journal: Journal of Chromatography A - Volume 1555, 22 June 2018, Pages 100-105
نویسندگان
Yan Zhang, Luqi Zhu, Yating Zhang, Pingang He, Qingjiang Wang,