کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7615086 | 1493982 | 2018 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development and validation of a method for gefitinib quantification in dried blood spots using liquid chromatography-tandem mass spectrometry: Application to finger-prick clinical blood samples of patients with non-small cell lung cancer
ترجمه فارسی عنوان
توسعه و اعتبارسنجی یک روش برای اندازهگیری ژفییتینیب در لکههای خشک شده با استفاده از اسپکترومتر جرمی کروماتوگرافی مایع: استفاده از نمونههای خون بالینی انگشتان دست و پا در بیماران مبتلا به سرطان ریه غیر سلولی
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantification of gefitinib in dried blood spots (DBSs). Gefitinib was extracted with methanol from DBS of 3â¯mm in diameter and detected using a triple quadrupole mass spectrometer. The method was validated by evaluating its precision, accuracy, selectivity, carryover, matrix effect, recovery, and stability. For clinical validation, paired finger-prick DBS and plasma concentrations were compared for 10 patients with non-small cell lung cancer (NSCLC) taking gefitinib. The calibration linear range was 37.5-2400â¯ng/mL (coefficient of determination [R2]â¯=â¯0.99), encompassing the therapeutic concentrations of gefitinib. The accuracy and precision were within 15% of the quality control (QC) concentrations of 80, 200, and 2000â¯ng/mL. The lower limit of quantification was determined to be 40â¯ng/mL. Gefitinib was stable in DBSs for up to 5â¯months at room temperature and â20â¯Â°C, and at 40â¯Â°C for 24â¯h. A good correlation was observed between the gefitinib levels measured by the DBS method and plasma concentrations (R2â¯=â¯0.99). This method provides a simple, fast, and accurate approach to the quantitative analysis of gefitinib in finger-prick DBSs. The method would be useful for minimally invasive evaluation of the clinical gefitinib blood concentration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 1087â1088, 15 June 2018, Pages 1-5
Journal: Journal of Chromatography B - Volumes 1087â1088, 15 June 2018, Pages 1-5
نویسندگان
Kei Irie, Saori Shobu, Seika Hiratsuji, Yuta Yamasaki, Shigeki Nanjo, Chiyuki Kokan, Akito Hata, Reiko Kaji, Katsuhiro Masago, Shiro Fujita, Yutaka Okada, Nobuyuki Katakami, Shoji Fukushima,