کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7615866 | 1493997 | 2017 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A rapid quantitative analysis of bile acids, lysophosphatidylcholines and polyunsaturated fatty acids in biofluids based on ultraperformance liquid chromatography coupled with triple quadrupole tandem massspectrometry
ترجمه فارسی عنوان
تجزیه و تحلیل کمی از اسیدهای صفراوی، لیسفسفاتیدیل شولین ها و اسیدهای چرب غیر اشباع در بیوفیلد ها بر اساس کروماتوگرافی مایع فوق العاده و همراه با سه بعدی چهارپروپول توده ی اسپکترومتری
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کلمات کلیدی
طیف سنجی جرم کروماتوگرافی مایع، تجزیه و تحلیل کمی، اسیدهای صفراوی، لیسفسفاتیدیل شولین، اسیدهای چرب اشباع نشده،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Much evidence suggested that quantitative analysis of bile acids (BAs), lysophosphatidylcholines (LPCs), and polyunsaturated fatty acids (PUFAs) in biofluids may be very useful for diagnosis and prevention of hepatobiliary disease with a non-invasive manner. However, simultaneously fast analysis of these metabolites has been challenging for their huge differences of physicochemical properties and concentration levels in biofluids. In this study, we present a liquid chromatography-mass spectrometry method with a high throughput analytical cycle (10Â min) to fast and accurately quantify fifteen potential biomarkers (eight BAs, four LPCs and three PUFAs) of hepatobiliary disease. The accuracy for the fifteen analytes in plasma and urine matrices was 80.45%-118.99% and 84.55%-112.66%, respectively. The intra- and inter- precisions for the fifteen analytes in plasma and urine matrices were all less than 20% and the lower limit of quantification (LLOQ) of analytes is up to 0.0283-8.2172Â nmol/L. Therefore, this method is fast, sensitive and accurate for the quantitative analysis of BAs, LPCs and PUFAs in biofluids. Moreover, the stability and concentration differences of the analytes in plasma and serum were evaluated, and the results demonstrated that LPCs is stable, but PUFAs is very unstable in freeze and thaw cycles, and the concentrations of the analytes in serum were slightly higher than those in plasma. We suggested plasma may be a kind of better bio-sample than serum using for quantitative analysis of metabolites in blood, due to the characteristics of plasma are more close to blood than those of serum.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 1068â1069, 15 November 2017, Pages 343-351
Journal: Journal of Chromatography B - Volumes 1068â1069, 15 November 2017, Pages 343-351
نویسندگان
Zhangxiao Peng, Qian Zhang, Ziming Mao, Jie Wang, Chunying Liu, Xuejing Lin, Xin Li, Weidan Ji, Jianhui Fan, Maorong Wang, Changqing Su,