کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7627103 | 1494584 | 2018 | 22 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential mobility spectrometry followed by tandem mass spectrometry with multiple ion monitoring for bioanalysis of eptifibatide in rat plasma
ترجمه فارسی عنوان
طیف سنجی تحرک دیفرانسیل و به دنبال آن طیف سنجی جرم دو طرفه با نظارت بر چندین یون برای بررسی زیست اپتیفیتادی در پلاسما موش
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Eptifibatide is a therapeutic cyclic peptide with poor collision-induced dissociation (CID) efficiency for multiple reaction monitoring (MRM), which limits the development of a traditional liquid chromatography-tandem mass spectrometry (LC-MS/MS) bioassay with MRM. In this study, a method combining differential mobility spectrometry (DMS) with liquid chromatography-multiple ion monitoring (LC-DMS-MIM) was developed for the quantitation of eptifibatide in rat plasma. After solid phase extraction (SPE) of 100â¯Î¼L plasma on an Oasis® HLB cartridge, the analyte and I.S. (octreotide) were analyzed using a SCIEX QTRAP 6500 operated in the positive ion mode and preceded by a DMS device. The lower limit of quantitation (LLOQ) for eptifibatide was 0.5â¯ng/mL using only 100â¯Î¼L plasma. The method was linear in the concentration range 0.5-300â¯ng/mL with good precision and accuracy. Compared to regulated quantitative LC-MS/MS bioanalysis of eptifibatide, the LC-DMS-MIM method effectively overcomes the sensitivity challenge in the LC-MRM method and reduces the high background noise and matrix interference in LC-MIM method without DMS. The method was successfully applied to a pharmacokinetic study involving intravenous injection of eptifibatide to Wistar rats.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 151, 20 March 2018, Pages 260-265
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 151, 20 March 2018, Pages 260-265
نویسندگان
Tianming Ren, Runzhi Li, Xiangjun Meng, J.Paul Fawcett, Dong Sun, Jingkai Gu,