Leukocyte p53 protein biosignature through standard-aligned two-dimensional immunoblotting

► Protein analyses of leukocytes are hypothesized to provide essential information regarding systemic diseases. ► Our p53 biosignatures detects subtle variations in p53 isoform expression. ► Our fluorescently labelled alignment standard dramatically improved comparison and analysis of 2-D immunoblots. ► Applying the alignment standard to p53 biosignature analysis led to the discovery of a putative novel p53 protein variant.