Subcellular fractionation of stored red blood cells reveals a compartment-based protein carbonylation evolution

► Different evolution of protein carbonylation in various erythrocyte subcellular fractions. ► Cumulative aspect of carbonylation during storage, particularly in cytoskeleton. ► Enrichment of carbonylated content in microparticles. ► Increasing loss of carbonylation in erythrocytes via microparticulation. ► Highlight of the potential role of cytoskeleton oxidation in microparticulation.