A label-free proteome analysis strategy for identifying quantitative changes in erythrocyte membranes induced by red cell disorders

► Direct proteome analysis of red cell ghosts was superior to ghost membrane extracts. ► Excellent depth of analysis with separation of red cell ghosts into 30 fractions ► Use of 1D SDS gels provided insight into protein size heterogeneity. ► Label-free quantitation methods to address unsolved questions in red cell disorders