کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7640116 | 1494853 | 2018 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Designing an electrochemical aptasensor based on immobilization of the aptamer onto nanocomposite for detection of the streptomycin antibiotic
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Streptomycin (STR), an aminoglycoside antibiotic, was used in human and veterinary to treat gram-negative infections. STR residue causes serious side effects on human health. Accordingly, to achieve a novel and sensitive method for detection of STR, we attempted to develop electrochemical sensing system based on graphene quantum dots (GQDs) functionalized with amine (NH) and thiol (SH) groups (GQDs-N-S) as the promising newest carbon-based nanomaterial. In this sensing system, gold nanoparticle (AuNPs) was deposited on the GCE surface. Then GQDs-N-S was coated on the AuNPs/GCE surface. After, the silver nanoparticle (AgNPs) coated on the GQDs-N-S/AuNPs/GCE. Finally, the STR thiol-aptamer (SH-Apt) was covalently attached on the AgNPs/GQDs-N-S/AuNPs/GCE via bonding formation of AgS. This system showed linear range from 0.01â¯pg·mLâ1 to 812.21â¯pg·mLâ1 with limit of detection of 0.0033â¯pg·mLâ1; it was as good as or better than other similar assays, and also the proposed system, in the presence of different interferences, displayed good selectivity in detecting STR. The proposed aptasensor showed excellent results in quantitatively the detection of STR in serum samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microchemical Journal - Volume 141, September 2018, Pages 96-103
Journal: Microchemical Journal - Volume 141, September 2018, Pages 96-103
نویسندگان
Mahmoud Roushani, Kazhal Ghanbari, S. Jafar Hoseini,