کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7681498 | 1495821 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A facile label-free electrochemiluminescence biosensor for target protein specific recognition based on the controlled-release delivery system
ترجمه فارسی عنوان
یک بیوسنسور الکتروکمیلومینسانس الکتروشیمیایی بدون علامت برای تشخیص خاص پروتئین هدف بر اساس سیستم تحویل کنترل آزاد
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
This paper described a novel label-free electrochemiluminescence assay for target protein based on a controlled delivery system. Iron oxide magnetic mesoporous silica nanocontainers were prepared by using a general procedure. The prepared magnetic mesoporous silica nanocontainers were applied to load the guest molecules [Ru(bpy)3]2+. Aptamers were used as gatekeepers on the pore outlets of the nanocontainers. In the presence of target proteins, the specific aptamer-protein interactions were employed as triggers for uncapping the pores and releasing the guest molecules from the nanocontainers. The amount of the guest molecule [Ru(bpy)3]2+ released from the magnetic mesoporous silica nanocontainers was monitored by the electrochemiluminescence assay. The results show that the releasing amount of [Ru(bpy)3]2+ is proportional to the thrombin concentration in the range of 0.6Â pM-0.8Â nM with a detection limit of 0.5Â pM (S/N=3). The present work demonstrates that the fabricated nanocontainer using aptamer as the cap is a highly sensitive and selective key-in lock gating system for the label-free ECL biosensor.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 114, 30 September 2013, Pages 5-10
Journal: Talanta - Volume 114, 30 September 2013, Pages 5-10
نویسندگان
Xiaoyan Yang, Aiguo Wang, Jilong Liu,