کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7704969 | 1496904 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Applications of calcium electroporation to effective apoptosis induction in fibrosarcoma cells and stimulation of normal muscle cells
ترجمه فارسی عنوان
استفاده از الکتروپورسیون کلسیم به القاء آپوپتوز موثر در سلول های فیبروسارکوم و تحریک سلول های عضلانی نرمال
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
الکتروشیمی
چکیده انگلیسی
The electroporation (EP) supports various types of anticancer therapies by the selective transport of cytostatics. Increase in intracellular calcium level by EP may be a new approach to fibrosarcoma treatment. Calcium is one of the most important factors of cell proliferation, differentiation and cell death (apoptosis or necrosis). Calcium level balanced by electroporation can cause different effects on normal and pathological cells. The efficiency and safety of electroporation combined with Ca2Â + ions were examined in our study. The two muscle cell lines were used: normal rat skeletal muscle cells - L6 and cancer muscle cells - Wehi-164 (fibrosarcoma). Two CaCl2 concentrations were tested: 0.5Â mM and 5Â mM combined with EP parameters: 1000Â V/cm, 1200Â V/cm, and 1500Â V/cm. The results show that EP supported by Ca2Â + is cytotoxic for Wehi-164 cells and simultaneously safe for normal muscle cells. The main type of cell death - apoptosis - was confirmed by Tunnel and Annexin V/PI assay. Additionally, sPLA2 pro-tumorigenic influence was proved by immunocytochemistry. Moreover, EP with 0.5Â mM of Ca2Â + slightly stimulates the normal muscle cells - L6 to increase proliferation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioelectrochemistry - Volume 109, June 2016, Pages 70-78
Journal: Bioelectrochemistry - Volume 109, June 2016, Pages 70-78
نویسندگان
Anna Zielichowska, MaÅgorzata Daczewska, Jolanta Saczko, Olga Michel, Julita Kulbacka,