Application of immobilized Rhodobacter sphaeroides bacteria in hydrogen generation process under semi-continuous conditions

► Application of VitraPOR®Filter porous glasses for Rhodobacter sphaeroides O.U. 001 bacteria immobilization. ► Application of new flat photobioreactor (170 ml) in hydrogen generation which was operating under semi-continuous conditions. ► Modification of porous glasses surface with AEAPTMS (3-(2-aminoethyl)aminopropyl)trimetoxysilane.